Coding
Sir4

Part:BBa_K106001:Design

Designed by: Ryan Quan   Group: iGEM08_UCSF   (2008-10-22)

Sir4, Aar1 AD part


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2554
    Illegal SpeI site found at 3670
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 385
    Illegal SpeI site found at 2554
    Illegal SpeI site found at 3670
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2192
    Illegal BamHI site found at 2516
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2554
    Illegal SpeI site found at 3670
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2554
    Illegal SpeI site found at 3670
    Illegal AgeI site found at 1887
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 392
    Illegal SapI site found at 1756


Design Notes

For more information on AarI cloning, please refer to the UCSF 2008 igem page.

Source

PCR'd from yeast genome in parts then ligated together.

References